|
Analytical Ultracentrifugation Membrane Confined Analytical Electrophoresis Complementary Analytical Techniques
|
|
Membrane-Confined Analytical
Electrophoresis
|
|
| The MCE apparatus is a unique instrument that
provides a means of determining the charge, electrophoretic mobility
and diffusion coefficient of macromolecules under a variety of
conditions. The charge of a molecule is a fundamental property that is
inextricably linked to its structure, solubility and interactions. MCE
is a primary method for measuring this charge directly and routinely,
without comparison to standards. Experiments under both steady state
electrophoresis and mobility electrophoresis can be performed. Presentation (pdf): The importance of accurately measuring protein valence. Applications: Quality control and analysis. Cataloging and characterization of charge on macromolecules. Determining the size, shape and hydration of macromolecules. Academic and industrial uses: 1) Detecting isozyme variants; 2) Measuring effects of different buffer conditions on ion binding; 3) Investigating charge variations of DNA; 4) Study of drug-DNA and protein-protein interactions. Sample Preparation: For information specific to your application, contact: Susan Chase, Supervisor, CAMIS Laboratory Technical Services E-mail: sfchase@cisunix.unh.edu Tel: (603) 862-1639 |
 Key features of the MCE: Small sample sizes (2-100 μg). Does not require a standard. Buffer can be changed without disrupting the sample. |
